Other studies had shown that within the pancreatic bud epithelium, the expression of the YY is limited to the endocrine cell pool and will not overlap the YY+ and PDX1 + cells which was identified before the positive cells hormone were elaborated. The expressing subset gave clues to the differentiation of the committed PDX1+ epithelial progenitors (Kahan et al
This article focuses on the importance of differentiating ? cells from ES as seen via the developmental stages in the pancreatic endocrine cells, definitive endoderm, and primitive foregut/gut tube. Considering the difficulties involved when handling human embryos and the difference between other species and human beings, differentiating human ES cells to pancrea allows for investigation of differentiating ES cells from the endoderm to the pancreases using a number of arrays proteomics (Lee & Chung,2011, p
The differentiation system we used therefore represents a protocol that directs the ES cells of the mouse into the pancreatic lineage by generating IPCs. It is applicable to other strategies which makes the vitro differentiate into the insulin-producing cells (Liu & lee, 2012, p
This suggests that there are factors in the pancreatic environment that makes the human cells to mature. (Murry & Keller, 2008, p
In this case, it was found that hESCs could be directed into the intestinal epithelium by just activating WnT and at the same time inhibiting Notch signaling. Also, when cultured, MEF and M15 and ESCs could further be differentiated into intestinal cells types (Ogaki et al
Subsequently, it was concluded that the differentiation resulted in the generation of the pancreatic lineage cells. (Pethe, Nagvenker & Bhartiya,20 14, p
From this, it was deduced that there are chances that the Pdx1-engineered ES cells could enhance the pancreatic lineage commitment that drives the ES cells into the IPCs that are responsive to glucose. The problem is to eliminate the partially differentiated stem cells (Raikwar & Zavazava, 2012, p